To investigate the role of USP5/METTL14 axis in glycolysis and RA-FLS activation, CCK-8 assays were performed, which showed that overexpression of METTL14 partially reversed the decrease in cell viability caused by USP5 knockdown in TNF-α-treated RA-FLSs (Fig. 4A). Here, TNF is linked to rheumatoid arthritis.