Comparison of the candidate binding partners of the four ARD constructs (WT, R269C, R315W, D333G) revealed 21 proteins that were identified as putative interactors of both the WT-ARD and D333G-ARD but were absent from the interactomes of both neuromuscular disease mutant ARDs (Fig. 5A, Table 2): GORAB, CETN3, CRACR2B, PAQR3, ERMN, TERF2IP, MOGAT2, ARHGEF10, ARFGAP1, RPL6, DFFA, SCOC, EIF3J, BDH2, ARL10, HYPK, SH3D19, FAM104B, LBHD1, KIRREL3, and RAB11FIP1. This evidence concerns the gene ARHGEF10 and neuromuscular disease.