Among the 62 differentially expressed genes, Immunoregulatory interactions between a lymphoid and non-lymphoid cell (z-score = 3.162, Fisher's exact p = 3.45e-11) and TCR signalling (z-score = 2.828, Fisher's exact p = 3.08e-10) were the top-two activated pathways with csACR, while CTLA4 signalling in cytotoxic lymphocytes (z-score = −2.111, Fisher's exact p = 6.21e-09) and PD-1, PD-L1 cancer immunotherapy (z-score = −1.342, Fisher's exact p = 4.94e-06) were the top negatively regulated pathways (Fig. 3). Here, PDCD1 is linked to cancer.