CASP1 and alkaline phosphatase measurement: In this study, the astragalin treatment markedly reduced the expression of NLRP3, P20-Caspase-1, ASC, and IL-1β proteins in the pancreatic tissue, whereas they were dramatically enhanced in the CAE group (Fig. 5), suggesting that activation of NLRP3 was manifested in AP, and astragalin administration had a depressant effect.