After 10 days, the MM model was successfully established observed by the IVIS imaging system, the mice were randomly divided into 4 groups and injected with PBS, free BTZ, BTZ@PLGA nanovesicles, BTZ@PLGA/EM nanovesicles (0.4 mg/kg BTZ), respectively for once every 3 days for a total of three doses. This evidence concerns the gene CASC3 and Miyoshi myopathy.