PDX models were established by co-transplanting 2.0×104 cells from each of the AAVS1, TET2 and TP53 nucleofected populations into NSGS immunodeficient mice with 1.0×105 CD34+ cells derived from either HD BM confirmed to be JAK2V617F-negative by ddPCR (control; n=4), JAK2-mutant MF (n=4), or JAK2-mutant PV (n=4) patients (Table S1). This evidence concerns the gene JAK2 and acquired polycythemia vera.