Furthermore, myeloid MR deficiency inhibited cardiac gene expression of Ctgf and also reduced accumulation of collagen in diabetic hearts based on both histological and immunostaining techniques, suggesting that myeloid MR signalling contributes to cardiac stiffness during diabetes by promoting cardiac fibrosis [24], which is consistent with previous studies of non-diabetic MR-dependent cardiac disease [11,13]. This evidence concerns the gene CCN2 and diabetes mellitus.