CCK-8 and EdU assay results demonstrated that PCa cells with STARD4 depletion were resistant to enzalutamide treatment, whereas increased STARD4 expression enhanced the suppressive effects of enzalutamide on the proliferation of both 22Rv1 and C4-2-EnzR cells (Fig. 8B-C). Here, STARD4 is linked to posterior cortical atrophy.