To elucidate the biological function of STARD4 in PCa, we overexpressed STARD4 in the 22Rv1 and C4-2 cell lines while employing shRNA to achieve stable STARD4 depletion in LNCaP, 22Rv1, and C4-2 cells (Figure S2A). This evidence concerns the gene STARD4 and posterior cortical atrophy.