While analyzing serum insulin levels, we focused on examining the effects of obesity on the expression of aromatase and insulin receptors in the epiphyseal growth plate.This study established a high-fat diet (HFD)-induced obese rat model to investigate the mechanistic links between obesity-related hyperinsulinemia, growth plate development, and local aromatase expression. Here, CYP19A1 is linked to obesity due to melanocortin 4 receptor deficiency.