These clusters were subsequently annotated based on canonical marker genes and were classified into the following cell types: keratinocytes from the stratum corneum (KC S. Corneum), regulatory T cells (Tregs), CD161+ T cells, CD8+ T cells, keratinocytes from the stratum spinosum (KC S. Spinousm), mature dendritic cells (Mature DCs), macrophages, keratinocytes from the stratum granulosum (KC S. Granulosm), CD4+ T cells, melanocytes, and keratinocytes from the basal layer (KC S. Basale) (Figure 2B). The gene discussed is CD4; the disease is keratoconus.