AR expression was potently reduced by recombinant TGFβ1 and significantly upregulated in C3/myCAF upon pharmacological TGFβR inhibition or YAP1 siRNA-mediated knockdown with a similar trend observed upon pharmacological YAP1 targeting implicating the concerted suppressive action of TGFβ and YAP1 in contributing to stromal AR loss in PCa. Here, TGFB1 is linked to posterior cortical atrophy.