To test this, we detected the activity of PI3K and AKT upon ALYREF depletion or PARP10 depletion, and the results revealed that either ALYREF depletion or PARP10 depletion significantly decreased the phosphorylation levels of PI3K and AKT in ovarian cancer cells (Fig. EV4A,B). This evidence concerns the gene AKT1 and ovarian cancer.