Immunoblotting analysis of lysates from cells of the above mentioned conditions showed consistent modulation of HK2 on the protein levels and further showed that modulation of HK2 by MCL1 is not shared with Bcl-xL or Bcl-2 and was observed in a panel of melanoma cells (Fig. 4B, C and Supplementary Fig. 5B, C). Here, BCL2 is linked to melanoma.