FC analysis further revealed that the combination of USP25 overexpression and anti-PD-1 therapy markedly reduced the accumulation of MDSCs, but strongly increased the number of tumor-infiltrating functional CD8+ T cells, including Granzyme B+ T cells, IFN-γ+ T cells, and TNF-α+ T cells (Fig. 7F). This evidence concerns the gene CD8A and neoplasm.