Immunoblot analyses of whole lysates of these ECs using an anti-mouse and anti-human lamin A antibody detected the shorter lamin A disease variant, progerin in both the LmnaG609G/+ and Prog-Tg cells, similar to a HGPS patient fibroblast sample, whereas it was absent in WT ECs (Fig 7D). This evidence concerns the gene LMNA and Hutchinson-Gilford progeria syndrome.