To investigate the transcriptome-wide effects of M protein-induced nuclear METTL3 depletion, we transfected A549 cells with either M protein expression plasmids or empty vectors, stimulated them with polyinosinic-polycytidylic acid (poly(I:C)) to mimic viral infection, and performed RNA-sequencing (RNA-seq) analysis. The gene discussed is METTL3; the disease is viral infectious disease.