KEGG pathway analysis revealed the top 10 significant pathways, including Cytokine-cytokine receptor interaction, Viral protein interaction with cytokine and cytokine receptor, Amoebiasis, IL-17 signaling pathway, Protein digestion and absorption, Rheumatoid arthritis, TNF signaling pathway, Chemokine signaling pathway, Neuroactive ligand-receptor interaction, and Hematopoietic cell lineage, as shown in Fig. 2E. The gene discussed is TNF; the disease is amebiasis.