To elucidate the mechanisms of Ast‐Por‐Rhe improving DKD, we quantified the relative expression levels of key proteins in the IRS1/PI3K/AKT signaling pathway, including IRS1, p‐PI3K, PI3K, p‐AKT and AKT, using western blot analysis. The gene discussed is IRS1; the disease is diabetic kidney disease.