The early and accurate detection of superoxide dismutase1 (SOD1)and other biomarkers is crucial for the diagnosis and monitoring ofneurodegenerative diseases such as amyotrophic lateral sclerosis.This study reports the development of a low-cost paper-based electrochemicalsensor for the detection of serotonin (5-HT) and an immunosensor forthe detection of SOD1, the potential biomarkers associated with thesediseases. Here, SOD1 is linked to amyotrophic lateral sclerosis.