As one of the main active phytochemicals in the traditional Chinese medicinal Shenjinhuoxue mixture, morusin contributed to the significant reduction of inflammatory mediators; Toll-like receptor 4 (TLR-4), IL-1 receptor-associated kinase 1, NF-κBp65, TNF-α, IL-6, IL-1β, receptor activator of NF-κB ligand (RANKL), and transient receptor potential vanilloid 1 (TRPV1) in the synovial and cartilage tissues of osteoarthritis (OA) rats following administration of the mixture (Ma et al., 2021). This evidence concerns the gene TLR4 and osteoarthritis.