To evaluate whether our Pin1 inhibitor, 164B8, can effectively target CAFs, we established primary normal fibroblast (NF) cultures from human peritoneum and primary CAF cultures from peritoneal metastases obtained from three individuals living with gastrointestinal cancer, including pancreaticobiliary, appendiceal, and colorectal cancer peritoneal metastases. The gene discussed is PIN1; the disease is colorectal cancer.