Next, knockout of USP25 using two specific CRISPR/Cas9 single guide RNAs (sgRNAs) led to a decrease in METTL3 protein levels, with no effect on METTL3 mRNA levels, in primary glioblastoma cells (GBM-85) and glioblastoma cell lines (U87-MG and U251) (Figures 3D and S4C). Here, METTL3 is linked to glioblastoma.