AKT1 and neoplasm: We then carried out single-sample GSEA (ssGSEA) for each tumor using the Hallmark PI3K_AKT_MTOR gene set to determine whether AKT signaling was increased in the T:E fusion–positive CRPC cases relative to the TCGA primary tumors, but we did not find a significant difference in the T:E fusion–positive or –negative tumors (Supplemental Figure 11B).