USP5 and nasopharyngeal carcinoma: In contrast, the knockdown of ATG5, which mediates LC3 lipidation, resulted in decreased p-STAT1/2 levels in LEVs from USP5-depleted NPC cells, which is consistent with the prior finding that LC3-dependent EV loading and secretion is ATG5 dependent (30, 31) (Supplemental Figure 8D).