Immunohistochemistry staining for CD44, TGF-β, and VEGFA1 was performed in lung tissues of A549 tumor-bearing mice, which revealed that regardless of RT, JMJD6-knockdown tumors had decreased positivity of CD44, TGF-β1 and VEGFA1 compared with control tumor (Fig. 3j). This evidence concerns the gene JMJD6 and neoplasm.