To preclude the possibility of indirect impacts of SMN on abnormal bone development, we conducted conditional knockdown of chondrocytic Smn1 (Smn1-cKD mice) by injection of AAV9-Col2a1p-shSmn1 into WT mice at P3 and daily record their weight until P12 (Fig. 7a). This evidence concerns the gene SMN2 and chronic kidney disease.