CD8+ cytotoxic T lymphocytes eradicate tumor cells through two cooperative mechanisms: the perforin/granzyme pathway induces rapid lysis of target cells, while secretion of cytokines such as IL-2, IFN-γ, and TNF-α establishes a proinflammatory milieu that sustains long-term cytotoxic activity [36]. The gene discussed is TNF; the disease is neoplasm.