The results of this study revealed that the independent risk factors affecting the overall survival (OS) of HCC patients included GGT > 50 U/L, macrovascular invasion, tumor diameter ≥ 10 cm, and number of tumors ≥ 3, among which the risk ratio (HR) for GGT > 50 U/L was as high as 1.78, with 95% confidence intervals (CIs) ranging from 1.26 to 2.50 and a p value of 0.001, which indicated that GGT > 50 U/L was associated with the overall survival rate of patients. The gene discussed is GGTLC3; the disease is neoplasm.