PRF1 and neoplasm: In intraperitoneal ID8VEGF tumor-bearing mice, the proportion of CD8+TILs from mice treated with TIM-3 and PD-1 inhibitors combination therapy was increased and showed the most active proliferation, the lowest TOX expression, and the highest TNF-α, IFN-γ, Perforin, and CD69 expression, compared to single treatment or isotype (Fig. 5B, C and Supplementary Fig. 5).