CD4 and neoplasm: The IF further verified the TIM-3 expression within the TME of HGSOC, as TIM-3 was predominantly co-expressed with markers, including CD4, CD8, and CD11c, while minimal expression was observed on pan-cytokeratin (CK) positive tumor cells, indicating that TIM-3 was predominantly expressed on CD4+T cells, CD8+T cells, and CD11c+ myeloid cells rather than tumor cells (Fig. 6B).