Furthermore, in the CCl4-induced liver fibrosis model, overexpression of NEK7 specific in hepatocytes delivered by AAV8-TBG vector still did not affect the levels of NLRP3 inflammasome activation in the livers, consistent with the findings in the CDAHFD model (Supplementary Fig. 11f–j). This evidence concerns the gene NLRP3 and Hepatic fibrosis.