In the present study, in CDAHFD-induced NAFLD mouse model, we found that the activation of NLRP3 inflammasome was not affected by overexpression of NEK7 in the liver via hydrodynamic injection of NEK7 plasmids, as evidenced by no significant difference in the expression of NLRP3 and caspase 1, the caspase 1 activity as well as the levels of mature IL-1β in livers (Supplementary Fig. 11a–e). The gene discussed is NEK7; the disease is metabolic dysfunction-associated steatotic liver disease.