Through measurements of intracellular reactive oxygen species (ROS), glutathione (GSH), malondialdehyde (MDA), and Fe2+ levels, along with the expression of ferroptosis-related genes [acyl-CoA synthetase long-chain family member 4 (ACSL4), NADPH oxidase 1 (NOX1), glutathione peroxidase 4 (GPX4), and heat shock protein 27 (HSP27)], it was demonstrated that PTBP1 knockdown significantly enhances ferroptosis in endometrial cancer cells. The gene discussed is GPX4; the disease is endometrial cancer.