LRP1 and neuroblastoma: Using murine BV2 (microglia) and N2a (neuroblastoma) clonal cell lines with Lrp1 deletion (KO) (15, 18), the Lrp1-deficient cells and their Lrp1-sufficient counterparts were infected with JCV (MOI = 0.1), and the amount of viral RNA in the supernatant at 24 hpi was measured by RT-qPCR (Fig. 1A).