Primary outcome was the Hamilton Anxiety Scale (HAMA), with secondary outcomes including Self-Rating Anxiety Scale (SAS), Generalized Anxiety Disorder 7-item scale (GAD-7), Pittsburgh Sleep Quality Index (PSQI), Self-Rating Depression Scale (SDS), cortisol (CORT), and adrenocorticotropic hormone (ACTH). The gene discussed is POMC; the disease is depressive disorder.