• Identifies cell populations in their respective spatial environment• Allows gene expression analysis in different cells, similar to scRNA-seq, but within tissue context• Reveals region-specific transcriptional remodelling• Maps cell-cell interactions between neighbouring cells• Identifies ligand-receptor pairs that can promote tumour progression or drive immune suppression, such as MDK-NCL• Stratifies tumours based on CD8+ T cell infiltration patterns• Offers insight into mechanisms of tumour resistance and sensitivity to treatments. This evidence concerns the gene CD8A and neoplasm.