TRPV4 and endothelial dysfunction: The role of TRPV4 in atherosclerosis is further supported by its functional links to endothelial dysfunction: shear stress enhances endothelial TRPV4 agonist sensitivity,37 and its activation increases ATP release, ROS production, and mitochondrial dysfunction.38,39 Our results were consistent with these studies, as elevated mitochondrial oxidative stress, reduced membrane potential, impaired autophagy, decreased respiratory function, and diminished ATP production were observed in ECs overexpressing TRPV4.