By integrating clinical sample analysis, multi-omics technologies (single-cell RNA sequencing, high-throughput sequencing), and in vitro and in vivo experimental models, it systematically investigated the mechanism of NOTCH1 in cervical cancer progression and radiosensitivity, and clarified the targeted regulatory relationship between has-miR-449a and NOTCH1. This evidence concerns the gene NOTCH1 and cervical carcinoma.