Next, we examined the effects of AAV-DYRK1A shRNA injection on AD-associated glial dynamics in 3.5-month-old 5xFAD mice and found that DYRK1A knockdown significantly reduced the mRNA levels of the RA markers GFAP, GBP2, CXCL10, and DST but not NESTIN (Figures 3D, E). Here, DST is linked to Alzheimer disease.