To delineate the expression profiles of shared diagnostic biomarkers (CPD, GGCT, SUZ12, ZMYM2) in mouse models of CHD and T2D, we performed multi-platform validation on aortic tissues using immunofluorescence (Figures 7A-D), Western blotting (Figures 7E-I), and RT-qPCR (Figures 7J-M). Here, GGCT is linked to type 2 diabetes mellitus.