Cleaning the injection site and adherence to aseptic techniques were both significantly associated with duration of diabetes (p < 0.001 for both), insulin therapy duration (p = 0.016 and p = 0.022, respectively), and age (p = 0.039 for cleaning; p = 0.081 for aseptic technique), whereas the device used did not show a significant effect in either case. The gene discussed is INS; the disease is diabetes mellitus.