To further elucidate the Ras‐PLD signaling pathway and autophagy mechanism in the treatment of NAFLD with quinoa, the mRNA levels of ras, Raf1, Mek, ERK, and Pld1 in liver tissue were evaluated by RT‐qPCR (Figure 7). The gene discussed is MAP2K7; the disease is metabolic dysfunction-associated steatotic liver disease.