We found that cg03651886 and SF1, Supplemental Digital Content, https://links.lww.com/MD/Q751 methylation (cg03986574) had a positive causal effect on MDD (FDR < 0.01), whereas DDA1 methylation (cg10664184) was negatively related to MDD (FDR < 0.01), as depicted in Figure 4 and ST-8, Supplemental Digital Content, https://links.lww.com/MD/Q751. This evidence concerns the gene DDA1 and major depressive disorder.