INS and Insulin resistance: Because palmitate-mediated insulin resistance in C2C12 myotubes is a commonly used model for assessing insulin resistance, this work aims to (1) highlight the concentrations and durations commonly used in palmitate-mediated insulin resistance in C2C12 myotubes; (2) describe the effects of palmitate-mediated insulin resistance in C2C12 myotubes on various outcomes associated with both basal and insulin signaling response; and (3) discuss the current obstacles and considerations associated with this model.