MYC and colorectal carcinoma: The specific objectives comprise: (1) evaluating the effects of EVO on the synthesis and metabolism of arginine in CRC cells, as well as its role in inhibiting cell proliferation and migration; (2) investigating the modulation of ASS1 expression by EVO via the Wnt/β-catenin/c-MYC signaling pathway; and (3) confirming EVO’s regulatory effects on the growth of CRC allografts and arginine metabolism by utilizing animal models, thereby offering experimental backing for potential clinical applications.