Therefore, the aim of the present study was to compare the results of all five commercially available Leptospira spp.-specific antibody POC tests in Germany (WITNESS® Lepto, Zoetis; FASTest® LEPTOSPIRA IgM, MegaCor; Test-itTM Leptospirosis, Life Assay; ImmunoComb® Canine Leptospira Test Kit, Biogal; and SNAP® Lepto, IDEXX) with the MAT results after vaccination using sera from 97 healthy, revaccinated dogs collected over 52 weeks. The gene discussed is CD40LG; the disease is leptospirosis.