However, it is surprising that, in the present study, only 32 out of 97 dogs were positive after two weeks, and 18 dogs were positive after four weeks in the IgM POC tests WITNESS® Lepto (Zoetis) (n = 18 and n = 10), FASTest® LEPTOSPIRA IgM (MegaCor) (n = 13 and n = 7), and Test-itTM Leptospirosis (Life Assay) (n = 1 and n = 1), respectively. This evidence concerns the gene CD40LG and leptospirosis.