Even at week 0, a marked discrepancy was observed between the tests that detect IgM antibodies (WITNESS® Lepto (Zoetis) and FASTest® LEPTOSPIRA IgM (MegaCor) with 1/97 positive results each, and Test-itTM Leptospirosis (Life Assay) all negative) and those POC tests that detect IgG antibodies, either exclusively (ImmunoComb® Canine Leptospira Test Kit (Biogal) with 32/97 positive) or in combination with IgM (SNAP® Lepto (IDEXX) with 13/97 positive). Here, CD40LG is linked to leptospirosis.