On the other hand, no polarization towards any response profile was observed with wtBCG-adjuvanted vaccine formulation (groups III and IV), and there was a slight polarization towards the Th1-type inflammatory response only when acute infection was present (group IV), which was probably the cause of some of the animals’ deaths, consistent also with a significant increase (** p ≤ 0.05) in IL-1β and IL-2 concentrations at the time of euthanasia vs. post-immunization/pre-infection time. This evidence concerns the gene IL1B and infection.