The estimation of allele and genotype frequencies of thrombophilia mutations (FV Leiden, FII, Methylenetetrahydrofolate Reductase (MTHFR C677T and MTHFR A1298C), and Plasminogen Activator Inhibitor-1 (PAI-1)) offers numerous advantages in different areas. The gene discussed is SERPINE1; the disease is thrombophilia.