GFAP and Alzheimer disease: To assess whether hippocampal astrocytes from 3×Tg-AD pups born to mothers with PEE exhibit increased inflammatory reactivity or astrogliosis, we analyzed the gene expression of inflammatory markers and markers indicative of glial reactivity: glial fibrillary acidic protein (Gfap), vimentin, tumor necrosis factor α (Tnfα), interleukins (Il1β and Il6), and prostaglandin-endoperoxide synthase 2 (Ptgs or Cox2).