Two-way ANOVA indicated effects of sex and PEE on Tnfα mRNA levels [F (1,20) = 4.666, p < 0.05; F (1,20) = 8.535, p < 0.01; respectively], with an overall increase in Tnfα mRNA levels in hippocampal astrocytes from ethanol 3×Tg-AD offspring of both sexes compared to respective control astrocytes (Figure 1C). The gene discussed is TNF; the disease is Alzheimer disease.