TNF and acute kidney injury: To further elucidate the role of ASFEE in AKI by regulating inflammation-related pathways, we evaluated the mRNA levels of TNF, IL-6, IL-1β, Rela (p65), Nfkbia, Cdkn1a (p21), Trp53, Mdm2, Pik3r 5, Rras2, Nras and Myc in the kidney tissues of mice in each experimental group by RT-qPCR method.