Silencing of Usp7 and Usp10 with their respective siRNA Usp7 #13 and Usp10 #14 at DIV 1 led to significant 44% and 42% reduction, respectively, of AD-TAU-induced seeded Tau aggregation in wildtype CTX (Figure 4A), indicating that Usp7 and Usp10 knockdown was equally effective in reducing seeded Tau aggregation in cortical neurons expressing mouse Tau harboring no mutations. This evidence concerns the gene MAPT and Alzheimer disease.