SOX2 and colorectal carcinoma: Immunohistochemistry (IHC) was performed on tissue microarray (TMA) slides comprising normal colon (n = 10), primary CRC (n = 40), and metastatic CRC (n = 10) samples to evaluate the correlation between CCN2 expression and the CSC markers SOX2 and Nestin (Nes) (Figure 7).